DNA
HR1-GAL1

Part:BBa_K300986:Design

Designed by: Susanna Zucca, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-03)

Homologous region in S288C yeast genome (GAL1 ORF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed this part to be homologous to the GAL1 ORF in the S. cerevisiae S288C genome.

In particular, this part is identical to the nucleotides from 31 to 75 of the reverse complement of the GAL10 ORF (locus tag: YBR020W - http://www.yeastgenome.org/).

This sequence does not show significant alignments with other genomic regions of S288C strain.

Source

S. cerevisiae S288C genome on the NCBI database.

References

[1] Kim MD, Lee TH, Lim HK, Seo JH (2004), Production of antithrombotic hirudin in GAL1-disrupted Saccharomyces cerevisiae. Applied Microbiology and Biotechnology Volume 65, Number 3 / August.

[2] West RW Jr, R. Rogers Yocum R, Ptashne M (1984), Saccharomyces cerevisiae GAL1-GAL1O Divergent Promoter Region: Location and Function of the Upstream Activating Sequence UASG. Molecular and Cellular Biology, November, P.2467-2478, Vol. 4. No. 11.

[3] Winzeler EA et al. (1999), Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis. Science 285, 901.